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500,000 Plasmids Shipped and Counting!

Posted by Caroline LaManna on Mar 26, 2015 11:24:00 AM

Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around the world in ~10 years!

We're extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on our mission – documenting your plasmids, storing them, providing quality control and customer support, making plasmids easy for you to find and request in our online collection, and shipping them to you quickly.

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Topics: Fun, Scientific Sharing, Inside Addgene

The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

Posted by Guest Blogger on Mar 10, 2015 10:25:06 AM

The following post was contributed by the Developmental Studies Hybridoma Bank.


Antibodies are the most widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists to target proteins of interest for labeling, quantification, purification, chromatin immunoprecipitation and more. The Developmental Studies Hybridoma Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over 3,000 antibodies and associated hybridoma cell lines.

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Topics: Scientific Sharing

Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Posted by Eric J. Perkins on Jan 15, 2015 8:50:00 AM

It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings in relatively isolated resorts creates a sense of comradery with fellow attendees from the moment you arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle, our driver regaled us with tales of back country skiing, fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome to Montana!

Though sadly I will not be attending the entire meeting, Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent keynote address was the first of 19 talks given over the course of the day. His talk, which focused on the history of genome engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed by three of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic community has clearly made a huge impact on this field.

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Topics: Genome Engineering, Scientific Sharing, Synthetic Biology, CRISPR

Addgene’s Top 10 Blog Posts from 2014

Posted by Caroline LaManna on Dec 16, 2014 3:01:00 PM

As 2014 comes to a close, we’ve been reflecting on the past year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it has grown steadily during 2014. We were excited to have more and more scientists offering to share their stories – about their research, their job hunts, and their tips for experiments. We’ve also loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding to your comments.

Additionally we’ve seen what topics are of interest to you, our readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our blog from the past year. A quick glance shows that interest in CRISPR continues to grow as the genome editing technique has developed and improved. The CRISPR posts written by our guest bloggers, the experts in the CRISPR/Cas field, have been extremely helpful for scientists that are looking for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists’ attention, and as we move into next year we’d like to know what topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating many additional questions. We’re happy to see scientists making their own cells to save money and create the tools that work best for their needs.

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Topics: Scientific Sharing, News

Make a Splash: Notions of Scientific Impact Are Evolving

Posted by Kendall Morgan on Dec 5, 2014 9:54:09 AM

Of course, all of you toiling away in laboratories this holiday season want the work you are doing to have an impact, to move science forward, or perhaps even society. One obvious way to do that is and has been to publish in journals with a high “impact factor,” a measure that dates back to 1975 and is based on the average number of citations for recent articles. Of course, a publication in Science or Nature is always nice, but in the wired world we are living in, there are plenty of other ways to define and measure scientific impact.

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Topics: Career, Scientific Sharing

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