Michelle Cronin

Michelle Cronin is an Addgene Senior Scientist, with a background in neurobiology, molecular biology, and biochemistry.

Recent Posts

Plasmids 101: FLEx Vectors

Posted by Michelle Cronin on Apr 28, 2016 10:30:00 AM

In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can be used to induce site-specific recombination events, and that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA fragment. The availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new, creative ways. Today’s post will focus on one such strategy--the FLEx switch--which utilizes recombination elements to turn off expression of one gene, while simultaneously turning on the expression of another!

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Topics: Plasmid Technology, Plasmids 101, Cre-lox

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