Using Phosphoserine to Study Protein Phosphorylation

Posted by Guest Blogger on Jun 23, 2016 10:30:00 AM

This post was contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin.

It is commonly cited that approximately one-third of cellular proteins are modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array of model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent than previously appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies a staggering ~250,000 phosphorylation events in the proteomes of higher mammals (2). How can we begin to understand the importance of any of these phosphorylation events on the activity of a given protein?

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Topics: Plasmid How To, Synthetic Biology, Lab Tips, Techniques

Evolution of Lab Techniques

Posted by Guest Blogger on Jun 21, 2016 10:30:00 AM

This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University.

Just as computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab are replaced by improved versions that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing classic papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their modern equivalents and see what we can learn from their evolution.

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Topics: Fun, Lab Tips, Techniques

Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

Posted by Tyler Ford on Jun 15, 2016 9:30:00 AM

As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we’re proud to announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu, both from the University of Health and Allied Sciences in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they plan to use plasmids from Addgene to advance their research.

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Topics: Inside Addgene, Investigator Feature

Introducing Addgene's Microbiology Pages

Posted by Jessica Welch on Jun 13, 2016 3:08:05 PM

Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology community. On our new curated Microbiology Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for microbial research.

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Topics: Inside Addgene, Microbiology

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Posted by Mary Gearing on Jun 9, 2016 10:30:00 AM

Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the world, and the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more!

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Topics: Plasmid Technology, Genome Engineering, CRISPR

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