Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

By Alfonso Timoneda

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Stereotypical UV absorbance spectra exhibited by DNA and other substances commonly used in DNA purification procedures.
Cartoon of Addgene's mascot Blugene
Comparison of shRNA and miRNA processing pathways.
Cartoon of CRISPR-associated transposase (CAST) integrating donor DNA into a genomic target site.
A cartoon plasmid at the center of the image has arrows leading to many possible host species: gram negative bacteria, gram positive bacteria, yeast, and mammalian cells.

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