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Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Posted by Guest Blogger on Oct 19, 2017 9:54:49 AM

This post was contributed by guest blogger Katrin Michel.

Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control.

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Topics: Plasmid Technology, Cre-lox, Techniques

Oh, The Places You Can Go: Careers in Science Communication - Product Development

Posted by Beth Kenkel on Oct 18, 2017 9:00:00 AM

In this post in the Careers in Science Communication blog series, you’ll learn about Caitlin Runne-Janczy, a Product Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum to support them. Caitlin’s interview is broken into two parts, with part one detailing how she got into scicomm and part two focusing on what her job at eScience Labs is like. Find all the posts in this series here.

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Topics: Career, Science Communication, SciComm Careers

3D Printing Meets CRISPR Cas9

Posted by Guest Blogger on Oct 17, 2017 8:55:24 AM

This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the CEO of 3-D Molecular Designs.

Have you ever held your favorite protein in the palm of our hand? Well, actually – have you ever held a model of your favorite protein in your hand? At 3D Molecular Designs, we create physical models of proteins using 3D printing technology. The tagline for our company is “…where molecules become real”.  We sometimes forget that the models we create are around 30 million times larger than the real thing. Nevertheless, the models are compelling, and I encourage you to hold one.

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Topics: CRISPR

Fluorescent Tagging of Endogenous Genes with SapTrap

Posted by Michelle Cronin on Oct 12, 2017 10:26:13 AM

Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function of cellular systems. FPs have been used to track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression of fluorescently tagged proteins can lead to improper protein localization, protein aggregation, or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role.

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Topics: CRISPR, Fluorescent Proteins

A Deep Dive into BioBuilder

Posted by Guest Blogger on Oct 11, 2017 9:44:02 AM

This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology into the hands of students and teachers in the U.S. and beyond.

“I want to learn more about Synthetic Biology. Can you tell me where to start?” is a question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology.”

These are the types of inquiries that routinely hit the inbox at the BioBuilder Educational Foundation. With a goal of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current questions and experiments in the field of synthetic biology.

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Topics: Synthetic Biology, Science Communication

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