Latest Posts

All Posts

Learning Technical Skills at a Lab Central Workshop

Posted by Racheal Komuhendo on Feb 20, 2018 9:30:07 AM

As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston area, we get many opportunities to learn from other biotech companies at workshops and can use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and discuss why these types of workshops could be useful to anyone working in a lab.

Read More >

Topics: Inside Addgene

New Videos: Addgene Lab Tips

Posted by Eddy Page on Feb 15, 2018 9:41:16 AM

Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience in processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way to ourselves so we decided to share some of our tips in our new series: Addgene Lab Tips!

Read More >

CRISPR Challenges: Standardization and Homology Directed Repair

Posted by Mary Gearing on Feb 14, 2018 9:36:24 AM

Blugene and I represented Addgene at the recent Keystone meeting on Precision Genome Editing with Programmable Nucleases. Check out #KSgenome on Twitter if you missed our live updates!

Read More >

Topics: CRISPR

CUT&RUN: A Improved Method for Studying Protein-DNA Interactions

Posted by Guest Blogger on Feb 13, 2018 9:51:55 AM

This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill.

Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations and makes it easier for you to map protein-DNA interaction with limited biological materials.

Read More >

Topics: Plasmid Technology, Techniques

Technologies Enabled by NanoLuc® Luciferase

Posted by Guest Blogger on Feb 8, 2018 7:17:02 AM

This post was contributed by Kyle Hooper at Promega.

Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could “clone by phone”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving access to useful research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when we share?

Read More >

Topics: Fluorescent Proteins

Blog Logo Vertical-01.png

Subscribe to Our Blog