Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many scientists have used the protocol for this plasmid, and if it still being used. After learning of it's continued popularity, I decided this protocol would be a worthy first contribution to the protocols.io community.
Did I mention that this protocol is being used around the world? In the last month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!