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Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Posted by Caroline LaManna on Sep 23, 2014 11:05:27 AM

Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many scientists have used the protocol for this plasmid, and if it still being used. After learning of it's continued popularity, I decided this protocol would be a worthy first contribution to the protocols.io community.

Did I mention that this protocol is being used around the world? In the last month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!

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Topics: Scientific Sharing

Data Freedom: The Expansion of Data Sharing in Research Publications

Posted by Guest Blogger on Aug 5, 2014 2:51:13 PM

This post was contributed by Jim Woodgett.


Public Library of Science (PLOS) created a stir earlier this year when it announced its data access and sharing policy. Since early March, the open access publisher has required authors to include a note as to where readers may locate data supporting the research reported in PLOS publications. The policy was not an overnight revelation, rather it was the result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm as the organization left open the question of how much data was necessary and reasonable. PLOS has since clarified their data sharing policy and recently announced that of the 16,000 manuscripts that had been processed since the declaration, only a small fraction (<1%) of authors have asked for advice about the scope of the policy. End of story? Not quite.

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Topics: Scientific Sharing

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Posted by Kendall Morgan on Jun 12, 2014 11:30:00 AM

Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer than a biologist. Their focus at the lab is on plant-pathogen interactions, and her aim is to produce constructs and edit genomes so as to make plants, and agricultural crops in particular, resistant to disease. They also devise biosensors designed to elucidate the molecular interactions that go on between plants and their pathogens.

As Patron explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the nucleus, or pathogens to their hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they work to overcome them, and why she spends some of her time engaging with others on Twitter, among other things.

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Topics: Plasmid Technology, Scientific Sharing, Synthetic Biology, Plant Biology, Plasmid Kits

London Calling - Addgene’s New Europe Office in London

Posted by Benoit Giquel on Jun 10, 2014 11:20:00 AM

You’ve called. You’ve emailed. And Addgene has been there to answer your plasmid questions. But until now, you’ve always had to call the United States - Cambridge, MA to be exact. Today, that changes.

Today, Addgene launches a brand new Europe office located in London and operated in collaboration with LGC Standards. And now you’ll be able to call us - Benoit Giquel and Emma Markham - the newest Addgenies. Got a question at 9:00am GMT? We’ll be poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts are still sleeping).

Plasmid Sharing in Europe: Addgene’s Request Statistics

Since 2004, Addgene has shipped more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the number of plasmids distributed to Europe has doubled going from 9,500 in 2010 to more than 20,000 in 2013. And the number of plasmids requested by scientists based in Europe over the first five months of 2014 reaffirms this trend.

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Topics: Scientific Sharing, Inside Addgene

10 Ways to Share Your Science!

Posted by Kendall Morgan on May 13, 2014 4:34:00 PM

You've heard it before  it's important for scientists to get out there and talk about their work. To tell others why that work is cool and why it really matters! There are important societal reasons, self-interested reasons, and, given that science is largely funded by tax-payers, there are reasons to feel obligated too, as experts on the subject brought out in a Kavli Foundation Round Table.

In any field, good communication skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative roles in academia, and that means it’s important to know how to talk about science to everyone – not just to others working in your own field. Alternative careers are now the norm for those (like myself) with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument for taking the time to hone your communication skills. So, where can a graduate student or post-doc start?

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Topics: Career, Scientific Sharing, Networking

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