CRISPR 101: Mammalian Expression Systems and Delivery Methods

Posted by Nicole Waxmonsky on Sep 24, 2015 10:30:00 AM


Experimental Possibilities for CRISPR
CRISPR technology has been widely adopted for genome editing purposes for numerous reasons including that it's cheaper, faster, and easier than prior editing techniques. With more and more CRISPR tools being published each month, you may be considering using CRISPR for your next experiment. In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical applications for each, and potential delivery methods.

 

 

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As with any experiment, there are many factors that need to be considered during the planning process. For CRISPR experiments, the following framework can help get you started:

  1. Determine the type of outcome you are trying to achieve: Do you want to permanently knock-out or knock-in a gene? Do you want to enhance or repress gene expression? Are you trying to create a single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components.
  2. Select the appropriate CRISPR tools for your application: Wildtype Cas9 or the Cas9 nickase are appropriate for knocking-in, knocking-out, or introducing mutations and tags, while a “dead” or dCas9 can be used in conjunction with activator or repressor domains to control gene expression.
  3. Choose an appropriate expression system and delivery method: Do you need stable integration or is transient expression sufficient? Which cell types will you be editing? Do you want to deliver the components as DNA or would mRNA or protein delivery be more suitable?
  4. Determine how you will evaluate the outcome: Will you be detecting insertions/deletions using a mismatch repair assay? Or is PCR followed by gel electrophoresis or Next Generation Sequencing more appropriate?

If you already have an end product in mind, steps 1 and 2 will generally be straightforward. Likewise for step 4, as this ties directly back to the specific application you have chosen. When thinking about step 3, however, you may be surprised at the number of options available--how do you choose?

One of the first steps is to identify what CRISPR components you will need to deliver. Minimally, one or more sgRNAs and Cas9 are required for any application. If you want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you will need to make sure your expression system and delivery methods are compatible with all your components. Next, consider the best form those CRISPR components should be in based on your model system. CRISPR reagents can be delivered via transfection, nucleofection, viral infection, or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression system, you can then choose the best method for introducing those CRISPR components into your target cells. 

Mammalian CRISPR Expression Systems

Each model system will have its best practices for efficient delivery of CRISPR components. If you are new to your model system, a good first step would be to consult the literature to see if anyone has published work with a protocol that would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum where you may be able to find information regarding your system of choice. The table below summarizes the various components included with each expression system as well as suitable applications.

Expression System Components of System Application
Mammalian expression vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used for gRNA. May contain reporter gene (e.g. GFP) to identify and enrich positive cells or selection marker to generate stable cell lines. Transient or stable expression of Cas9 and/or gRNA in a mammalian cell line that can be transfected at high efficiency.
Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive cells. Packaging and Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines. Useful for difficult to transfect cell types and can be used in vivo. A common choice for conducting genome-wide screens using CRISPR/Cas9.
AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable expression of SaCas9 and/or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery.
Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 mRNA and gRNA, then delivered to target cells (e.g. microinjection or electroporation). Transient expression of CRISPR components, expression decreases as RNA is degraded within the cell. Can be used for generating transgenic embryos.
Cas9-gRNA riboprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient expression of CRISPR components, expression decreases as gRNA and Cas9 protein are degraded within the cell.

 

Delivery Methods for Mammalian Cell Lines

As mentioned above, the expression system you choose in many ways dictates the best method for introducing those CRISPR components into your target cells. DNA delivery into mammalian cell lines is quite broad and includes several different methods, so we've further broken down this category by common cell types and recommended delivery methods below:

 

Method Transformed cell lines (HeLa, HEK 293) Stem cells (hES, iPS) Primary cells (fibroblasts, epithelial cells)
 Transfection
  • lipid-mediated
  • cationic polymers
  • calcium phosphate
+    
 Electroporation
  • nucleofection
+ +  
 Viral delivery
  • lentivirus
  • retrovirus
  • adenovirus
  • AAV
+   +

This table is not inclusive of all methods, nor are these methods limited exclusively to in vitro cell culture. The user should review the current literature about their preferred model. 

If your expression system is not well characterized in terms of CRISPR use, you will want to invest some time in optimizing and testing the efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR testing tools:

Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome repair by non-homologous end joining (NHEJ) or HDR.

EGFP validation of sgRNAs: Can evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter.

Target DNA reporter system: Can evaluate component delivery with validated tools.


 Special thanks to Joel McDade for creating the Expression Systems table. Marcy Patrick Contributed to the writing of this post.

Interested in more CRISPR? Addgene is updating our popular CRISPR Guide Resource so be on the lookout for that in early October! 

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Topics: Genome Engineering, CRISPR, CRISPR 101

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