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RNA Tools

Recent Posts

Plasmids 101

Plasmids 101: Expressing shRNAs for RNA Interference

Alyssa Shepard June 17, 2025

An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most importantly, effective. In the research world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR ...

Comparison of shRNA and miRNA processing pathways.
RNA Tools

All in a Twist: dsRNA

Susanna Stroik February 2, 2023

Transcription, translation, and prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA – right?

Exogenous sources of dsRNA
Plasmids

RNA Interference in Plant Biology: New Tools for an Old Favorite

Guest Blogger October 27, 2020

This post was contributed by guest blogger, Robert Orr, who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function (LOF) experiment functions as the building block of our understanding of complex biological ...

Plasmids

RaPID Detection of RNA-protein Interactions

Beth Kenkel August 16, 2018

Sometimes it feels like DNA and protein get all the attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID), and that’s ...

Figure 1

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