This post was written by Angelo Nicolaci, a PhD student at Moffitt Cancer Center.
Turn it on? Turn it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression control tools as well as ...
It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: Option #1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several ...
The central dogma in molecular biology is DNA→RNA→Protein. To synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most ...
In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually well suited for recombinant protein expression. Many challenges can arise when ...
