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An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most importantly, effective. In the research world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR ...
Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications. But how does one invent a tool for applications that don’t even exist yet? Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just ...
Annotation of genes in immune cells typically involves the creation of germline knockout mice, which is time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been ...
If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may be time, then, to look towards genetic code expansion (GCE): ...
What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study gene expression in cells. But these reporters have limited use in vivo because they are based on light which is very easily scattered by tissue. Ultrasound, on the ...
Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection of plasmids for coronavirus research, including a handful of plasmids that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids, ...
This post was contributed by guest blogger, Robert Orr, who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function (LOF) experiment functions as the building block of our understanding of complex biological ...
