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Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This type of cell labeling technique involves the introduction of a transgene construct containing fluorescent proteins (XFP) of different colors to label an organ or entire ...
The central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These functions and others are controlled by collections of neurons that are intricately wired into circuits through synaptic connections (Shepherd, 2004). ...
Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists alike). My only in-depth exposure to neuroscience prior to this conference was a rotation in ...
This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated possibilities for ...
This post was contributed by guest blogger Derek Simon.
This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week are only half of the battle when using optogentics to answer a research question. The flip side of the optogenetics coin is ...
CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control gene expression, ...
