This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids in our repository with known reference sequences, such as full plasmid sequences ...
We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols and tips that you can use in your ...
