This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes are controlled by ...
This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing reactions a week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting the most out of the data ...
This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids in our repository with known reference sequences, such as full plasmid sequences ...
We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining the transformation step. Read on to find protocols and tips that you can use in your ...
