Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free expression systems, mammalian cells, yeast, flies, and even plants! In this blog we will review common ...
If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may be time, then, to look towards genetic code expansion (GCE): ...
This post was contributed by Sreepadmanabh M, a member of the iGEM team from IISER Bhopal. Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in the SynBio arena this year with a fresh team of twenty excited undergrads. ...
For the last 14 years, scientists have been coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes the repository a ...
Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit Giquel. Traveling from our offices just outside of London, it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial ...
This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated with low morbidity and mild symptoms. This changed in ...
This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics program and researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based state machines in living cells” (1), ...
This post was contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins are modified through phosphorylation (1). However, the expansion ...
