In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell types and each one requires different elements for vector propagation. This post, along ...
A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive ...
Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe. The ...
