Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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Cartoon depictions of SHERLOCK and DETECTR nucleic acid detection methods.  SHERLOCK can be used to detect dsDNA or RNA. The material is amplified using RPA (for dsDNA, producing more dsDNA) or RT-RPA (for RNA, producing cDNA). Either way, T7 transcription then produces RNA from the amplified material. A target RNA sequence is specifically recognized by Cas13, which then promiscuously cleaves nearby RNA reporters, freeing a fluorescent marker from a linked quencher.  DETECTR is used to detect DNA. The material is amplified using RPA, and a target DNA sequence is specifically recognized by Cas12a, which then promiscuously cleaves nearby ssDNA reporters, freeing a fluorescent marker from a linked quencher.

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