By Angela Abitua
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It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: Option #1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several ...
Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’t “broken,” their production does require continued animal ...
