This post was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing Scientific Manuscripts. Any scientific manuscript will require numerous other references to ...
Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed to make a DNA double strand break (DSB) in the genome ...
This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to help ...
The following post was contributed by the Developmental Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists to target proteins of interest for labeling, quantification, ...
The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the ...
This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our career series. “The only healthy communication style is assertive communication.” - Jim Rohn, Author, Entrepreneur, Motivational Speaker Do you have a difficult supervisor, or do ...
This post was contributed by Theresa Liao of the University of British Columbia. When transitioning from an academic science career path to a non-academic one, one of the biggest changes (and perhaps challenges) is the need to present yourself using a resume. Indeed, instead of ...
This post was contributed by Kurt Thorn of the Nikon Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires multiple ...
