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Alyssa Neuhaus

Alyssa is a Quality Control Scientist at Addgene. She received her master's degree from the University of Vermont studying avian malaria parasites. She loves discovering cool research happening in labs around the world and making science approachable.

Blog articles by Alyssa Neuhaus

CRISPR

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Alyssa Neuhaus November 14, 2024

You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering what the rest of our genome could possibly be made up of. The answer is… drum roll please… non-coding RNAs! You probably didn’t see that coming, right? Non-coding RNAs ...
Alt text: A schematic illustration of the CRISPR/Cas9-based approaches mentioned above. The first illustration (A) shows a DSB made at a transcription start site upstream of an exon. The second illustration (B) shows a DSB made at splice sites. The third illustration (C) shows a DSB made upstream and downstream of an exon to fully remove a genomic fragment. The fourth illustration (D) shows a DSB made within an exon to insert a synthetic polyadenylation signal. 
Plasmids 101

Plasmids 101: Broad Host Range Plasmids

Alyssa Neuhaus April 11, 2024

Understanding the characteristics of natural plasmids and how they function in nature can inform us on how to create better recombinant vectors. In this blog post, we’ll define plasmid host range, identify a few of the features that confer broad host range in plasmids in nature, ...
Graphic showing a narrow range host plasmid with one ORI, and a broad range host plasmid with three ORIs with variable inserts.
Viral Vectors

Viral Vectors 101: Inverted Terminal Repeats

Alyssa Neuhaus October 19, 2023

Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among the most important are the inverted terminal repeats (ITRs). ITRs are the ...
Structure of a recombinant AAV vector, where two ITRs flank the gene cassette.

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