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Marcy Patrick

Marcy is the Director of Production at Addgene and oversees the plasmid intake, storage, and QC processes. She loves that her job allows her to support the scientific community day in and day out, while still keeping up to date on all the cool science out there.

Blog articles by Marcy Patrick

Plasmids 101

Plasmids 101: Methylation and Restriction Enzymes

Marcy Patrick June 30, 2016

Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized product from a site-directed mutagenesis experiment? The answer ...
Plasmids 101

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Marcy Patrick October 29, 2015

Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and ...
Fluorescent Proteins

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Marcy Patrick May 15, 2014

Bioluminescence and fluorescence from proteins such as Green Fluorescent Protein (GFP) has likely existed in creatures such as jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and deduce its biochemical properties. Now GFP ...
Plasmids 101

Plasmids 101: Mammalian Vectors

Marcy Patrick March 25, 2014

Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian cells. Of course, these mammalian vectors must be compatible with the cell type they are tranfected into – a ...
Yeast

Plasmids 101: Yeast Vectors

Marcy Patrick February 25, 2014

In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenence within an E. coli cell, but vectors can be widely utilized across many different cell types and each one requires different elements for vector propogation. This post, along ...
Plasmid Elements

Plasmids 101: Antibiotic Resistance Genes

Marcy Patrick January 30, 2014

Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means of ...

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