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Plasmids 101: Restriction Cloning

By Tyler Ford

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Recent Posts

Plasmids 101

Plasmids 101: Golden Gate Cloning

Mary Gearing August 27, 2015

Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method has its own pluses and minuses, but Golden Gate cloning has been especially useful within both the synthetic biology and genome engineering fields. We’ll walk you through how ...

Synthetic Biology

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Kendall Morgan June 12, 2014

Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer than a biologist. Their focus at the lab is on plant-pathogen interactions, and her aim is to produce constructs and edit genomes so as to make plants, and ...

Plant Biology

Quick, Versatile Plant Transgenesis with GreenGate Plasmids

Kendall Morgan January 23, 2014

A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive ...

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