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Hannah Dotson

Hannah is a Senior Scientist on the Scientific Support Team at Addgene. Their PhD work at Boston University focused on developing small molecule-controlled tools for synthetic biology. Hannah enjoys helping researchers find and share their scientific tools and resources.

Blog articles by Hannah Dotson

Viral Vectors

Pooled Library Amplifications

Hannah Dotson May 30, 2024

If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid diversity, so ...
Cartoon depicting factors to consider for a pooled library when creating an amplification protocol; details are in text of link.
CRISPR Pooled Libraries

Viral Vectors 101: Preparing Pooled Libraries

Hannah Dotson July 13, 2023

If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through ...
Graphic showing flow chart of pooled library preparation.

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