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Top Requested Plasmid of 2017 - pMD2.G

Posted by Tyler Ford on Jan 10, 2018 11:13:59 AM

Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Identifying Sequence Elements with SnapGene's Feature Database

Posted by Guest Blogger on Dec 21, 2017 9:06:58 AM

This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene.

SnapGene was created to meet a need. While there were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these tools inadequate for planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences or properties of the DNA molecules they were using.

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Topics: Plasmid Technology, Blog

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Posted by Guest Blogger on Dec 14, 2017 9:08:09 AM

This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital.

A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop and use patient-specific somatic cells that are otherwise difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro.

Find stem cell plasmids at Addgene

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Topics: Plasmid Technology, Hot Plasmids, Stem Cells

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Posted by Guest Blogger on Oct 19, 2017 9:54:49 AM

This post was contributed by guest blogger Katrin Michel.

Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control.

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Topics: Plasmid Technology, Cre-lox, Techniques

AAVs CREATed for Gene Delivery to the CNS and PNS

Posted by Beth Kenkel on Sep 28, 2017 10:01:35 AM

Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain. Neurons of the peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge.

Find the PHP Plasmids Here!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

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