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Plasmids 101: An Inside Look at NGS Plasmid Quality Control

Posted by Amanda Hazen on Aug 3, 2017 8:49:45 AM

All plasmids coming through Addgene’s doors are now verified by next-generation sequencing (NGS) to provide you with more data. For most plasmids, we are now able to confirm the full insert and backbone sequence. In order to manage and interpret these data, we’ve updated our quality control workflow.  Here’s a peek at how our process has improved now that we’re backed by the power of NGS!

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Topics: Inside Addgene, Plasmids 101

Improved Plasmid Maps Powered by SnapGene

Posted by Tyler Ford on Jun 29, 2017 9:06:40 AM

In meetings, in surveys, on Twitter - there is one thing we've heard over and over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking, we’re excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive feature library, our updated plasmid and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just how much things have improved, check out the example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial launch so stay tuned for more updates.

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Topics: Inside Addgene, Using Addgene's Website

Addgene's a Nonprofit? Nonprofit Awareness Day 2017

Posted by Joanne Kamens on Jun 5, 2017 11:19:13 AM

When you think of a "nonprofit" organization what do you think of? Maybe the term brings to mind a social service organization like the Red Cross or the American Cancer Society, or maybe you think about a local food pantry or community arts organization. Many people are surprised to learn that Addgene is officially filed, recognized and operated as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit the public, not private interests. On this nonprofit awareness day, we layout the ways in which we promote our mission and work to enable researchers around the world.

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Topics: Inside Addgene

Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

Posted by Caroline LaManna on May 12, 2017 8:58:59 AM

Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy tool for you.  

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Topics: Scientific Sharing, Inside Addgene

The Power Behind NGS Plasmid Validation: seqWell

Posted by Guest Blogger on Apr 19, 2017 11:25:29 AM

This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc.

Plasmids and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs, as the relatively low cost and quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA, as well as the advent of extremely fast, cheap and accurate sequencing (“reading”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many labs. However, the high costs of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes like plasmid validation.

At seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids from all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using this technique in our partnership with Addgene to sequence large numbers of plasmids.

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Topics: Plasmid How To, Inside Addgene

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