The beginning of this century has seen some major advances in light microscopy, particularly related to the neurosciences. These developments in microscopy coupled with techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail. One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic resolution for studying colloidal gold (1). The method was based on using a thin plane (sheet) of light generated by sunlight to observe single gold particles with diameters less than 4nm.