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PEI Calculator for Planning AAV Packaging Transfections

Posted by Beth Kenkel on Jan 23, 2018 9:04:28 AM

As the saying goes, “failing to plan is planning to fail.” Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture dish and number of dishes used. Here’s to making AAV packaging a little easier!

Click here to download the PEI calculator for AAV packaging

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Topics: Viral Vectors

Top Requested AAV of 2017: pmSyn1-EBFP-CRE

Posted by Tyler Ford on Jan 17, 2018 9:57:12 AM

We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested AAV that became available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming online!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Top Requested Plasmid of 2017 - pMD2.G

Posted by Tyler Ford on Jan 10, 2018 11:13:59 AM

Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

A Novice's Perspective on Neuroscience - SfN2017

Posted by Tyler Ford on Dec 15, 2017 9:07:01 AM

Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists alike). My only in-depth exposure to neuroscience prior to this conference was a rotation in Qiao Zhou’s lab in graduate school where I worked on a project aiming to dedifferentiate mouse brain cells into neural stem cells. I was quite unsuccesful (although I definitely enjoyed the lab!) and would therefore consider myself a neuroscience novice (newb). In this post, I’d like to highlight some of the things that popped out to me as a novice and indicate why other neuroscience newbies might want to get into the field.

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Topics: Viral Vectors

AAV Titers: Where do they come from and what do they mean?

Posted by Leila Haery on Nov 15, 2017 10:00:00 AM

Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category by listing off things that fall into that category? Okay, let’s play! I’ll list the examples and you try to guess the category:

ELISA...
qPCR...
Digital droplet PCR...
DNA dot blot...
Transduction assay...
SDS-PAGE...
Electron microscopy…

Any guesses?

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Topics: Viral Vectors

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