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Top Requested AAV of 2017: pmSyn1-EBFP-CRE

Posted by Tyler Ford on Jan 17, 2018 9:57:12 AM

We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-hM4D(Gi)-mCherry was the top requested AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested AAV that became available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming online!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Top Requested Plasmid of 2017 - pMD2.G

Posted by Tyler Ford on Jan 10, 2018 11:13:59 AM

Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab!

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Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Identifying Sequence Elements with SnapGene's Feature Database

Posted by Guest Blogger on Dec 21, 2017 9:06:58 AM

This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene.

SnapGene was created to meet a need. While there were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these tools inadequate for planning, visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set experiments back days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the 21st century, many molecular biologists didn’t know the complete sequences or properties of the DNA molecules they were using.

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Topics: Plasmid Technology, Blog

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Posted by Guest Blogger on Dec 14, 2017 9:08:09 AM

This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital.

A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop and use patient-specific somatic cells that are otherwise difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro.

Find stem cell plasmids at Addgene

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Topics: Plasmid Technology, Hot Plasmids, Stem Cells

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Posted by Guest Blogger on Oct 19, 2017 9:54:49 AM

This post was contributed by guest blogger Katrin Michel.

Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single level of control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control.

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Topics: Plasmid Technology, Cre-lox, Techniques

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