Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per plasmid. We generally create kits to allow you to easily use a set of plasmids together on a tight budget.
Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be sure to come by booth #851 and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology community. On our new curated Microbiology Collection page, you can browse for plasmids by species and find our guides, plasmid kits and other resources for microbial research.
When cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable but fleeting.
“What’s been shown by others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties like growth, survival, and differentiation.”
CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple gRNAs. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options and cloning methods, but please scroll down for plasmids suitable for other model systems, including E. coli, plants, Drosophila, and zebrafish!
At Addgene we're continually impressed with the amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all of them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at firstname.lastname@example.org and maybe we can write a blog post about it!