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Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

Posted by Matthew Ferenc on Feb 13, 2014 8:00:00 AM

There are no universal rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can quickly and easily identify what your plasmid contains and other important information.

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Topics: Plasmid How To, Lab Tips, Plasmids 101

Plasmids 101: Antibiotic Resistance Genes

Posted by Marcy Patrick on Jan 30, 2014 10:29:00 AM


Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which cells successfully received the correct plasmid, scientists would spend hours to days trying find their correct clones. Additionally, the presence of a plasmid is disadventageous from the bacterium's perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once – it allows a scientist to easily detect plasmid-containing bacteria when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid. Viva la (bacterial) resistance! 

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Topics: Plasmid How To, Plasmid Elements, Lab Tips, Plasmids 101

Save Time and Money by Making Your Own Competent Cells

Posted by Melina Fan on Jan 28, 2014 10:10:58 AM

We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining the transformation step.

Read on to find protocols and tips that you can use in your own lab.

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Topics: Plasmid How To, Inside Addgene, Lab Tips

Three Tips to Organize Your Lab Notebooks in the New Year

Posted by Caroline LaManna on Jan 2, 2014 10:44:00 AM

Welcome to 2014! Get excited, it’s a new year. A time for resolutions: to wake up and get to the lab earlier, to read that stack of papers on your desk, and to tackle some new experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips to help you get organized and boost your productivity in 2014.

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Topics: Lab Tips

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