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Trends in CRISPR and SynBio Technologies [Slideshare]

Posted by Joanne Kamens on Feb 4, 2015 10:58:00 AM

Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections on the program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and distirbution for these fast moving fields. 

Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are in a unique position to observe and quantify how new technologies are being disseminated through the scientific community.

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Topics: Hot Plasmids, Genome Engineering, Inside Addgene, Synthetic Biology, CRISPR

We've Launched Addgene's New & Improved Website!

Posted by Caroline LaManna on Nov 19, 2014 10:10:53 AM

We have lift off! You may have noticed a new friendly face on our website this morning announcing the launch of our new website. We are excited to finally bring you our new and improved website. We’ve been working diligently over the past month to design a site that is easier to navigate and helps you more effectively find the plasmids (and plasmid cloning information) that you’re looking for. 

I’ve put together a few screenshots below to show you just some of our new features, but the best way for you to see what’s changed is to head over to the upgraded Addgene website and explore for yourself. Don’t worry – it’s the same URL you’ve been using for years, so I promise you won’t get lost!

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Topics: Inside Addgene, News

Countdown to Halloween @Addgene

Posted by Caroline LaManna on Oct 29, 2014 1:26:27 PM

Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3 things: fun, teamwork, and competition. And our Halloween celebration this Friday will combine all of these.

Addgenies have been organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, though I suspect that our costume contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers. Our Halloween costume contests have become an annual tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (pop culture events of the past year obviously make for good costumes), science and our plasmid collection (what science has been hot this year and how do you creatively turn it into an outfit?), and of course there’s always nostalgia (old board games and movies can provide great character costumes).

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Topics: Fun, Inside Addgene

Inside Addgene's 10 Year Anniversary Gala

Posted by Guest Blogger on Jul 3, 2014 11:48:00 AM

Hey guys, Blugene here!

As Addgene’s mascot, I’ve watched Addgene grow into the successful, internationally renowned plasmid repository that it is today. Although I wish I could take all of the credit, the success of the company is attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens, and founders, Melina Fan, Ken Fan and Benjie Chen. Last week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow and spread our mission. 

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Topics: Fun, Inside Addgene

6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

Posted by Lianna Swanson on Jun 26, 2014 10:23:15 AM

This blog was originally published on BitesizeBio here.

As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing reactions a week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting the most out of the data you get back from your sequencing runs.

When you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder. These controls help you properly visualize your results.The most important of those is to always look closely at the trace file (or chromatogram) of the sequencing results you get back from your favorite sequencing facility.

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Topics: Plasmid How To, Inside Addgene, Lab Tips

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