Latest Posts

All Posts

Hot Plasmids Episode 4: B. subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

Posted by Tyler Ford on Dec 11, 2017 9:02:49 AM

In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage.

Read More >

Topics: Hot Plasmids, Optogenetics, CRISPR, Podcast

Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

Posted by Tyler Ford on Oct 25, 2017 9:00:00 AM

In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage.

Read More >

Topics: Hot Plasmids, Optogenetics, CRISPR, Podcast

In Memoriam - The Origins of pBABE and Generations of Cancer Research

Posted by Guest Blogger on Sep 29, 2017 12:44:40 PM

This post was contributed by guest blogger, Jay P. Morgenstern,  Ph.D., Director, Genetic Engineering & Molecular Biology at Warp Drive Bio. The post is in memoriam of his late father and pBABE’s namesake, Harold (Babe) Morgenstern.

Read More >

Topics: Hot Plasmids, Cancer

AAVs CREATed for Gene Delivery to the CNS and PNS

Posted by Beth Kenkel on Sep 28, 2017 10:01:35 AM

Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain. Neurons of the peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge.

Read More >

Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

Posted by Tyler Ford on Sep 8, 2017 3:52:44 PM

In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our quarterly newsletter or on our hot plasmids webpage.

Read More >

Topics: Hot Plasmids, Podcast

Blog Logo Vertical-01.png

Subscribe to Our Blog