By Aliyah Weinstein
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If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be happy to learn that many DNA-based reagents are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents permeate the molecular biologist’s ...
This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for light microscopy-based neuronal reconstruction. To ...
Autophagy (Greek for “self-eating”) is a process by which cytoplasmic material, including organelles, are targeted to lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis, delivery of materials to be degraded to the lysosome, and ...
This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent probe. ...
Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at least one site. Phosphorylation is particularly important for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing ...
This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent protein database As recognized by the 2008 Nobel Prize, fluorescent proteins (FPs) have become one of the most indispensable tools in ...
This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could ...