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CRISPR 101: Mammalian Expression Systems and Delivery Methods

Posted by Nicole Waxmonsky on Sep 24, 2015 10:30:00 AM

This post was updated on Dec 4, 2017.

CRISPR technology has been widely adopted for genome editing purposes because it's cheaper, faster, and easier than prior editing techniques. More and more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!

In this blog post we’ll provide an overview of some CRISPR mammalian expression systems, the typical applications for each, and potential delivery methods.

 

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Topics: Genome Engineering, CRISPR, CRISPR 101

Genome-wide Screening Using CRISPR

Posted by Joel McDade on Aug 18, 2015 10:30:00 AM

This post was updated on Dec 8, 2017.

What genes are important in your phenotype of interest? Many scientists study diseases for which the underlying genetic cause is not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional experiments investigating the role of individual genes or entire pathways in a particular disease process. While CRISPR is certainly not the first means to carry out so-called “forward genetic screening experiments”, it is certainly the most robust. In this blog post, we will discuss how CRISPR libraries are being used to perform genome-wide screens and highlight some of the reagents that have been made publicly available through Addgene. 

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Topics: CRISPR, pooled libraries

Addgene Welcomes John Doench to the Advisory Board

Posted by Tyler Ford on Aug 13, 2015 10:30:00 AM

The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce that we’ve added John G. Doench, PhD to the Advisory Board. John Doench is Associate Director of the Genetic Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding, curation, and explanation of our CRISPR resources. We recently sat down with Dr. Doench for an interview to help further introduce him to the Addgene community.

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Topics: Interview, Inside Addgene, CRISPR, Podcast

Mapping the 4D nucleome with CRISPR/Cas9

Posted by Mary Gearing on Aug 11, 2015 10:30:00 AM

It seems that there’s a new CRISPR advance or technique published every week! One of the newest applications is a colorful system that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems can be used to label loci, such as fluorescence in situ hybridization (FISH) or fluorescently labeled TALEs, CRISPR/Cas9’s ease of use and ability to label live cells make this system truly advantageous. This new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization varies across the life of a cell, or how organization may be altered in disease states.

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Topics: CRISPR, Imaging, Fluorescent Proteins

CRISPR 101: Validating Your Genome Edit

Posted by Melina Fan on Jul 30, 2015 10:30:00 AM

You’ve created your gRNA expression construct and used Cas9 to introduce it into your target cells. Hooray! You’re ready to begin reading out data, right? Almost. In this blog post we’ll explain how to verify that your cells were appropriately edited. We’ll also cover the basic techniques for detecting insertion, deletion, and mutation events.

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Topics: Genome Engineering, CRISPR, CRISPR 101

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