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Plasmids 101: E. coli Strains for Protein Expression

Posted by Julian Taylor-Parker on Feb 10, 2015 10:06:00 AM

In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli for DNA propagation. While great for cloning purposes, these E. coli strains are not usually well suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein in E. coli. We will review the potential pitfalls of recombinant protein expression and some of the most popular commercial strains designed to avoid them.

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Topics: Lab Tips, Plasmids 101

Management for Scientists: Giving Feedback

Posted by Joanne Kamens on Feb 6, 2015 9:46:00 AM

This is the third in a 5 part series on Management for scientists. Subscribe to the Addgene Career Advice Posts here.

Feedback is help and it should result in authentic assistance for the recipient to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect of being a successful manager. Great bosses tell people where they stand clearly and routinely. They are clear with each employee about what they do well and where they need to improve, and they’re also clear about how the person is doing overall. Employees hate to wonder what you think of their work.

 Check out Joanne's Reddit AMA

 

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Topics: Career, Management for Scientists

Trends in CRISPR and SynBio Technologies [Slideshare]

Posted by Joanne Kamens on Feb 4, 2015 10:58:00 AM

Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections on the program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and distirbution for these fast moving fields. 

Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus, we are in a unique position to observe and quantify how new technologies are being disseminated through the scientific community.

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Topics: Hot Plasmids, Genome Engineering, Inside Addgene, Synthetic Biology, CRISPR

Career Coaching for Scientists: Why and Where Do I Find One?

Posted by Joanne Kamens on Jan 29, 2015 8:25:00 AM

Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current job situation. What can a coach do that is different from an advisor, boss or mentor? First, professional coaches have experience and knowledge to help scientists transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic infrastructure are not always as effective in mentoring for this transition. Second, a coach will help you set goals and then hold you accountable for carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience more seriously and I have seen, without exception, that this helps people do a better job at reaching their goals. 

Check out Joanne's Reddit AMA

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Topics: Career, Career Readiness

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Posted by Guest Blogger on Jan 27, 2015 10:13:47 AM

This post was contributed by Jordan Ward who is a postdoctoral fellow at UCSF.

Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us to knock out sequences and introduce mutations and epitopes with unprecedented ease. In the last year, several advances in C. elegans genome editing using CRISPR/Cas9 have emerged, which I will describe below. These new C. elegans approaches rapidly enrich for editing events without the need for any selective marker to remain in the edited animal. To my knowledge these approaches have not yet been extended to other organisms/cell lines, though it is likely that many aspects will broadly improve editing efficiency.

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Topics: Plasmid Technology, Genome Engineering, CRISPR

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