Latest Posts

All Posts

Fluorescence Microscopy Techniques - Which is Best for Me?

Posted by Guest Blogger on Oct 10, 2017 9:57:00 AM

This post was contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and a Lecturer on Molecular and Cellular Biology at Harvard University.

No matter whether you are a sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures of life; the key to a great image is contrast. The human visual system relies primarily on contrast to identify individual objects and perceive the world around us. Without contrast, objects simply vanish into noise.

Read More >

Topics: Fluorescent Proteins

Tips for Working in Industry & Success in Management: Interview with Crystal Shih

Posted by Tyler Ford on Oct 5, 2017 9:13:38 AM

Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed in your own career. One pro tip from Crystal - be agile and willing to move on to new projects when old ones prove unsuccessful.

Read More >

Topics: Career, Management for Scientists, Career Readiness, Podcast

Oh, The Places You Can Go: Careers in Science Communication - Science Writing

Posted by Beth Kenkel on Oct 3, 2017 8:11:55 AM

This series was written for selfish reasons: I wanted to learn about careers in science communication. When I started my Science Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog pieces. Throughout my internship, my interest in scicomm has grown and now it feels like an awesome bionerd hobby but also a viable away-from-the-bench career option. So if you’re interested in learning more about science communication careers, you’re in the right place. For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit industries.

Read More >

Topics: Career, Science Communication, SciComm Careers

In Memoriam - The Origins of pBABE and Generations of Cancer Research

Posted by Guest Blogger on Sep 29, 2017 12:44:40 PM

This post was contributed by guest blogger, Jay P. Morgenstern,  Ph.D., Director, Genetic Engineering & Molecular Biology at Warp Drive Bio. The post is in memoriam of his late father and pBABE’s namesake, Harold (Babe) Morgenstern.

Read More >

Topics: Hot Plasmids, Cancer

AAVs CREATed for Gene Delivery to the CNS and PNS

Posted by Beth Kenkel on Sep 28, 2017 10:01:35 AM

Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the lack of a method for genetically manipulating neurons throughout the whole brain. Neurons of the peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction efficiency, none allow for simple and efficient transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge.

Read More >

Topics: Plasmid Technology, Hot Plasmids, Viral Vectors

Blog Logo Vertical-01.png

Subscribe to Our Blog