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Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Posted by Mary Gearing on Nov 24, 2015 10:30:00 AM

At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein even more useful! From FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things. Now, work from Connie Cepko’s lab allow researchers to activate transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations, saving the time and money needed to develop new lines.

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Topics: Plasmid Technology, Synthetic Biology, Fluorescent Proteins, Cre-lox

Illuminating Epigenetics with A FRET Based Biosensor

Posted by Emma Markham on Nov 19, 2015 10:30:00 AM

Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature. So what is epigenetics and what tools are available to study it?

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Topics: Hot Plasmids, Fluorescent Proteins, Techniques

Networking and Inspiring Young Scientists at Freshers Fairs

Posted by Emma Markham on Nov 17, 2015 10:30:00 AM

Have you ever heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative events are one of the main ways budding young scholars across Europe get introduced to the cultures and opportunities available to them at university.

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Topics: Career, Fun, Networking

MassBioEd Career Exploration Day at Addgene

Posted by Jessica Welch on Nov 13, 2015 10:30:00 AM

Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, Joanne had plenty of advice for the group of juniors and seniors, including that it’s never too early to start networking!

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Topics: Career, Networking, Career Readiness

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Posted by Joel McDade on Nov 12, 2015 10:30:00 AM

This post was updated on Dec 5, 2017.

Diverse genomes and genomic targets require a variety of tools to engineer them effectively. Since the discovery and engineering of dCas9, the CRISPR toolbox has expanded to include a variety of natural and engineered Cas proteins. Read on to learn how these tools can be used to expand CRISPR's reach to new genomic loci. 

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Topics: Genome Engineering, CRISPR

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