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CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Posted by Joel McDade on Jan 19, 2016 10:57:10 AM

The advent of CRISPR/Cas9 made it easier than ever to efficiently make precise, targeted genome modifications. Cas9 has been modified to enable researchers to knock out, activate, repress or even image your favorite gene.  But, with such a wide variety of Cas9-based reagents available, how do you choose which Cas9 is right for your particular experiment?  This blog post will help familiarize you with the wide array of Cas9s available through Addgene’s repository and make it easy to select the Cas9 reagent that is right for you.

The first thing to do in any CRISPR experiment is identify what, exactly, you are trying to do.  Are you trying to permanently knock-out a gene in a cell type or organism? Are you trying to reduce expression of a particular gene without permanently modifying the genome?  Does it make more sense to try and activate at a particular locus?  What about modifying the epigenome at a particular location?  As you might expect, the answer to this question will substantially affect your decisions about which Cas9 you need for your experiment.  Below is a brief summary of a few of the common genetic manipulations one can carry out using Cas9 and the specific Cas9s that can be used for each.

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Topics: CRISPR, CRISPR 101

Addgene's Tips for Plasmid Quality Control

Posted by Julian Taylor-Parker on Jan 14, 2016 10:30:00 AM

One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence in the plasmids we distribute through our quality control processes. Every plasmid we receive is rigorously verified before becoming available to the community.

This is no small task, however, at a repository that consistently receives around 200 new DNA samples every week. Here we will provide an inside look at the steps we take to verify the identity and quality of the plasmids we make available and provide some advice on the steps you can take to verify your own plasmids.

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Topics: Plasmid How To, Inside Addgene, Lab Tips

Finding Your Perfect Job After University

Posted by Emma Markham on Jan 12, 2016 10:30:00 AM



Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different organisations, so I tend to provide them with an overview of my experience and how I felt about the different positions to enable them to decide for themselves.  Here is my experience looking for the perfect job.

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Topics: Career, Career Readiness

How to Deposit Your Plasmids with Addgene

Posted by Tyler Ford on Jan 5, 2016 10:30:00 AM

At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute them to scientists at academic and non profit institutions worldwide. What's more, depositing is free! When we travel to talk to scientists about how we can help them share plasmids, we get many questions about how the deposit process works. To make things a little easier for you, we've written this post as a step-by-step guide to the online deposit process.

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Topics: Inside Addgene

Your Top Requested Plasmid in 2015!

Posted by Tyler Ford on Dec 30, 2015 10:30:00 AM

We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...

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Topics: Fun, Inside Addgene, CRISPR, pooled libraries

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