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An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

Posted by Tyler Ford on Dec 8, 2015 10:30:00 AM

We recently sat down with Addgene Board Member, depositor, and Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko's lab and to get some insight into her management and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-activated proteins, read our recent blog post.

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Topics: Interview, Viral Vectors, Podcast

Addgene's Top 10 Blog Posts of 2015

Posted by Tyler Ford on Dec 7, 2015 1:00:00 PM

2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent to the research community, two eBooks (Management for Scientists and the Plasmids 101 2nd ed), and have started providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth with our readership expanding from ~10,000 users per month in November 2014 to ~30,000 users per month in November 2015.

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Topics: Fun

Plasmids 101: Stringent Regulation of Replication

Posted by Jason Niehaus on Dec 3, 2015 10:30:00 AM

Plasmids utilize their host cell's replication machinery in order to replicate. As described in our previous Origin of Replication post, DNA replication is initiated at the ORI and may be synchronized with the replication of the host cell's chromosomal DNA or may be independent of the host's cell cycle. 

Plasmids are said to be under stringent control of replication when they are dependent on the presence of initiation proteins synthesized by the host cell in order to start their own replication. In general, these types of plasmids tend to be low copy number. Conversely, plasmids that can initiate DNA replication independently of the host's initiation proteins are said to be under relaxed control, as they only require the host's replication machinery for elongation and termination. These types of plasmids tend to be high copy number.

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Topics: Plasmid Elements, Plasmids 101

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Posted by Joanne Kamens on Dec 1, 2015 10:30:00 AM

Having never been to China myself, I was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s no wonder it was attended by over 300 Chinese scientists eager to hear current findings in nucleic acid science. I was delighted to see that the majority of attendees were scientists still working at the bench. It was a fantastic opportunity to talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10 countries in number of plasmid requests from Addgene.

Check out Joanne's Reddit AMA

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Topics: Scientific Sharing, Inside Addgene

#DeckTheLab to Win a Tasty Winter Treat This Holiday Season

Posted by Jane Hannon on Nov 30, 2015 10:30:00 AM

Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to decorate the lab but his short arms are causing him some difficulty…

To give him some inspiration, we’d love to see how you’re decorating your lab for the upcoming wintertime festivities!

Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays and tweeting it with the tags #DeckTheLab and @Addgene. Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it. 

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Topics: Fun

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