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Your Top Requested Plasmid in 2015!

Posted by Tyler Ford on Dec 30, 2015 10:30:00 AM

We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...

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Topics: Fun, Inside Addgene, CRISPR, pooled libraries

28 Hot Plasmid Technologies from 2015

Posted by Tyler Ford on Dec 23, 2015 10:30:00 AM



At Addgene we're continually impressed with the amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all of them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it!

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Topics: Plasmid Technology, Hot Plasmids, Plasmid Kits

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Posted by Mary Gearing on Dec 17, 2015 10:30:00 AM

If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does this system not use site-specific recombination, it also doesn’t require a ligation step! Based on the robust system of homologous recombination found in E. coli, SLIC is a cheap, standardized, and rapid multi-part DNA assembly method - read on to learn how to use it in your research.

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Topics: Plasmid How To, Plasmids 101, Protocols, Plasmid Cloning

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Posted by Tyler Ford on Dec 16, 2015 10:30:00 AM

As evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on research and will be affecting our everyday lives very soon. Not only is CRISPR having effects on various biological disciplines, the base technology itself is constantly improving. Cas9 variants have been modified for genome editing, activating gene expression, visualizing genomic loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target specificity of the Cas9 nuclease with engineered variants: eSpCas9SpCas9-HF1, & HypaCas9.

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Topics: Genome Engineering, CRISPR

A Better Way to Get Customer Support: The Help Center

Posted by Bryan Archambault on Dec 10, 2015 10:30:00 AM

Addgene’s Customer Service Philosophy

Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When you finally speak to a human being, you may not even get a solution to the problem you called about. Next, you try an email, given that this is the 21st century and all. But while the auto-reply telling you your message was received is a nice touch, it doesn’t help much if the actual response takes 3 days and still doesn’t even answer your question.

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Topics: Inside Addgene

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