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'Tis the Season to #DeckTheLab

Posted by Jane Hannon on Dec 2, 2016 10:30:00 AM

Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again this year!

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Topics: Fun

Plasmids 101: Knockout/Knock-In Plasmids

Posted by Benoit Giquel on Dec 1, 2016 10:30:00 AM

One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept is simple: deliver a template with a modified version of the targeted sequence to the cell which will recombine the template with the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain valuable, especially in cases where CRISPR cannot be used (e.g. there are no suitable PAM sequences nearby or your gene of interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy!

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Topics: Plasmid How To, Genome Engineering, Plasmids 101

Bricking Science: Portraying Scientific Reality Through LEGO

Posted by Guest Blogger on Nov 29, 2016 10:30:00 AM

This post was contributed by guest blogger, Dalila Cunha de Oliveira.

Bricking Science is an idea built, literally, 'brick-by-brick' to introduce people all around the world to the lives of researchers and PhD students.

Everybody in science knows that there are many ways your experiments can go wrong. Whether it be a bad fridge freezing your samples, or a dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes no culprit can be found…. In our lab we call this mysterious source of failure the lab gnome.

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Topics: Fun, Scientific Sharing, Science Communication

Tips for Improving Your Next Manuscript

Posted by Guest Blogger on Nov 22, 2016 10:30:00 AM

This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses with the American Society for Microbiology (ASM) Scientific Writing and Publishing Institute.

Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and share them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely to have an impact because it will be difficult or (more likely) impossible to find; therefore all the time spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer.

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Topics: Scientific Sharing, Career Readiness

10 Great Guest Posts We're Thankful For

Posted by Tyler Ford on Nov 18, 2016 10:00:00 AM

It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started!


Thanks for all your hard work!


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Topics: Fun

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