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Plasmids 101: Environmental Plasmids

Posted by Jessica Welch on Jan 31, 2017 10:30:00 AM

Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy in research, but where did these tools come from and why do they exist in nature? Read on to learn more about environmental plasmids, and how they’ve helped us develop molecular biology tools for the lab.

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Topics: Plasmids 101, Microbiology

Plasmids: Simplified

Posted by Tyler Ford on Jan 20, 2017 10:26:04 AM

We recently worked with YouTuber, Tom McFadden, to create a Plasmid Rap and introduce newcomers to the world of plasmids. As part of this process, we sent Tom a primer on plasmids and some of the ways that they can be used. We present this primer to you now with the hope that you can use it to introduce plasmids to any novice molecular biologist. You can find much more information about plasmids and their uses in our Plasmids 101 series. Happy reading!

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Topics: Plasmids 101

Plasmids 101: Gateway Cloning

Posted by Maria Soriano on Jan 12, 2017 10:30:00 AM

When facing a cloning project, scientists are no longer limited to traditional restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given set of resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into a variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages.

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Topics: Plasmids 101, Plasmid Cloning

Your Top Requested Plasmid in 2016!

Posted by Tyler Ford on Jan 11, 2017 3:47:00 PM

2016 was an exciting year for genome engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of these technologies were without controversy, and it remains to be seen how popular any one of them will become. One thing is for sure, as is obvious from our most requested plasmid, SpCas9 is still going strong as the basis for many genome editing experiments. So, without further ado, the most request plasmid in 2016 was...

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Topics: Fun, Inside Addgene, CRISPR, pooled libraries

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Posted by Guest Blogger on Jan 11, 2017 10:30:00 AM

This post was contributed by guest blogger, Gary McDowell, executive director of Future of Research.

On December 1st 2016, many postdocs working more than 40 hours per week could expect to see their salary raised to at least a new legal minimum of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was due to the threshold at which salaried workers receive overtime payment for working more than 40 hours per week increasing from $23,660 to $47,476 per year. This post discusses how a nationwide injunction against the FLSA is affecting universities' decisions to alter postdoc salaries - in some cases reversing these decisions entirely.

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Topics: Career

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