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Supporting Rare Disease Research with BeHeard 2017

Posted by Tyler Ford on Jul 20, 2017 10:30:00 AM

We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can improve your heart health. These are amazing advances that will surely save thousands if not millions of lives, but many throughout the world are affected by devastating diseases that affect far fewer people and therefore fail to garner much international attention. Their small numbers do not, however, make these patients suffer any less acutely. 

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Topics: Blog

Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

Posted by Susanna Bachle on Jul 18, 2017 8:41:28 AM

Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing countries. We are proud to introduce the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects spanning from the identification of plant compounds and insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides as well as developing yeast as source for dietary supplements!

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Topics: Scientific Sharing, Investigator Feature, Seeding Labs

DIY DNA Ladders from Penn State University

Posted by Beth Kenkel on Jul 14, 2017 10:30:00 AM

Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike many commercially available ladders, the 100 bp ladder works well for both agarose and native polyacrylamide gels.

Get Tips on Verifying Your Plasmid

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Topics: Hot Plasmids, Lab Tips

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Posted by Guest Blogger on Jul 13, 2017 10:30:00 AM

This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University.

Do you have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila genome? Continue reading and I’ll show you how Drosophila can be used to push your research in new and exciting directions.

Drosophila are very easy to manipulate genetically and have limited genetic redundancy (meaning, there’s more of a chance of seeing a phenotype since additional genes that can do the same function are less likely to exist). If there’s an ortholog of your favorite gene (YFG) in Drosophila (and even if there’s not!) the wealth of Drosophila genetic tools available allow you to study many aspects of your gene’s functional biology in a living organism. This is the first post in a three-part series. We’ll first discuss how to get started on fly work in this post. The second post will detail a major tool used by Drosophila geneticists (the Gal4/UAS system), and the third post will describe how you can make your own mutant flies.

Find Drosophila Resources at Addgene

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Topics: Lab Tips, Drosophila, Quick Guide to Drosophila

Custom CRISPR Screens & the Green Listed Software

Posted by Guest Blogger on Jul 11, 2017 10:30:00 AM

This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden.

It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green Listed software can help you do just this, probably in less than a minute (1).

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Topics: CRISPR

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