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Tyler Ford

Tyler J. Ford is an Outreach Scientist at Addgene. His professional duties include helping maintain the Addgene blog (blog.addgene.org), talking to people about Addgene, and improving Addgene's services. His non-professional duties include running, biking, drawing, hiking, playing tennis, reading, and writing.
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Recent Posts

Plasmids 101: Restriction Cloning

Posted by Tyler Ford on Feb 18, 2016 10:42:06 AM

When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.

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Topics: Plasmids 101, Protocols, Plasmid Cloning

Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

Posted by Tyler Ford on Jan 21, 2016 11:52:39 AM

In the third installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his graduate work in Professor Jim Collins' lab (then at Boston University, now at MIT) where he worked on understanding bacterial antibiotic resistance. During this time, Dr. Koeris and Professor Timothy Lu (a graduate student in the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain however, the time was not right for the development of phage-based therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6.

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Topics: Career, Interview, Career Readiness, Podcast

How to Deposit Your Plasmids with Addgene

Posted by Tyler Ford on Jan 5, 2016 10:30:00 AM

At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with us and distribute them to scientists at academic and non profit institutions worldwide. What's more, depositing is free! When we travel to talk to scientists about how we can help them share plasmids, we get many questions about how the deposit process works. To make things a little easier for you, we've written this post as a step-by-step guide to the online deposit process.

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Topics: Inside Addgene

Your Top Requested Plasmid in 2015!

Posted by Tyler Ford on Dec 30, 2015 10:30:00 AM

We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015 was...

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Topics: Fun, Inside Addgene, CRISPR, pooled libraries

28 Hot Plasmid Technologies from 2015

Posted by Tyler Ford on Dec 23, 2015 10:30:00 AM



At Addgene we're continually impressed with the amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all of them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it!

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Topics: Plasmid Technology, Hot Plasmids, Plasmid Kits

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