If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique and relies on specific components that are key to the cloning reaction. Understanding the specific components is essential for choosing the correct cloning method for your own experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what role does it play in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning experiments a little easier.
Cancer – a term familiar to almost every person regardless of profession, race, or gender. Almost no other disease receives as many billions of dollars in funding or as much attention collectively from researchers. Scientists, however, have yet to slay the beast. On a global scale, tens of millions of new cancer cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make new cancer resources easily accessible to the research community.