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Marcy Patrick

Marcy is a Senior Scientist at Addgene. She received her PhD in Microbiology and Immunology from the University of Michigan. She loves that her job allows her to interact with scientists from all over the world and help them easily share their reagents.

Recent Posts

Plasmids 101: Methylation and Restriction Enzymes

Posted by Marcy Patrick on Jun 30, 2016 10:30:00 AM

Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from a PCR reaction but not the newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction digests.

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Topics: Lab Tips, Plasmids 101, Plasmid Cloning

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Posted by Marcy Patrick on Oct 29, 2015 10:30:00 AM

Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything from parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part to the fact that very little was known about the role these factors played within an organism. 

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Topics: Plasmids 101, Plasmid Cloning

CRISPR 101: A New Series on Genome Editing & CRISPR-Cas

Posted by Marcy Patrick on Mar 5, 2015 12:06:12 PM

I am sure by now you have heard of CRISPRs. (If not, you can get up to speed here and here and here.) With such a fast moving technology, it is sometimes hard to keep pace with the new advances let alone remember the (maybe) long forgotten details of the biological process required to effectively design and utilize these tools. We certainly understand and are here to help!

Starting next week, we'll release the first post in our newest blog series - CRISPR 101 - a companion series to our popular Plasmids 101 articles. These posts are created to educate all levels of scientists and provide a resource for some of the basic principles driving CRISPRs and genome editing technology.

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Topics: Genome Engineering, Plasmids 101, CRISPR, CRISPR 101

ICYMI: Addgene’s Plasmids 101 eBook

Posted by Marcy Patrick on Oct 2, 2014 1:25:00 PM

We'd like to thank all of the contributing Addgenies that made this eBook possible: Melina Fan, Matthew Ferenc, Larissa Haliw, A. Max Juchheim, Caroline LaManna, Margo Monroe, Kendall Morgan, Jason Niehaus, Marcy Patrick, Lianna Swanson, Julian Taylor-Parker

We'd also like to thank our guest contributor: Gal Haimovich of greenfluorescentblog.org for helping us explain why things glow!

Addgene's Plasmids 101 eBook is here: Enjoy more time developing clever experiments and less time researching basic plasmid features – download the Addgene Plasmids 101 eBook!

Our goal was to create a one-stop reference guide for plasmids. We’ve combined our Plasmids 101 blog posts from the last year with some additional resources to create one downloadable PDF you can save to your desktop for easy reference. Highlights include our guide to fluorescent proteins, information about promoters and ORIs, and tips for naming your plasmids.  

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Topics: Plasmid Elements, Lab Tips, Plasmids 101

Plasmids 101: Viral Vector Elements

Posted by Marcy Patrick on Jul 17, 2014 3:09:00 PM

The use of viral vectors in research is beneficial for a number of reasons, including but not limited to: helping to get difficult-to-deliver DNA into mammalian cells, increasing the efficiency of gene transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification. At the most basic level, viral vectors consist of a viral genome that has been adapted into a plasmid-based technology and modified for safety through the removal of many essential genes and the separation of the viral components. Read on for a brief description of the viruses used to make these vectors as well as a table defining the major elements found within the plasmids comprising the viral vector systems.

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Topics: Plasmid Elements, Plasmids 101, Viral Vectors

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