A few years ago, researchers introduced what’s been called a major breakthrough in cloning technology with the Golden Gate method. For the first time, it was possible to quickly and efficiently assemble a large number of building blocks with just two or three inexpensive enzymes. Now, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems.
“The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on the daily needs of an advanced molecular plant science lab.”