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Jessica Welch

Jessica Welch is an Outreach Scientist at Addgene. She received her PhD in Microbiology from the University of Queensland and moved to Boston from Australia in 2011. She would love to see Addgene's mission of scientific sharing spread to researchers all over the world.

Recent Posts

MassBioEd Career Exploration Day at Addgene

Posted by Jessica Welch on Nov 13, 2015 10:30:00 AM

Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, Joanne had plenty of advice for the group of juniors and seniors, including that it’s never too early to start networking!

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Topics: Career, Networking, Career Readiness

Tips for Screening with Yeast Two Hybrid Systems

Posted by Jessica Welch on Oct 22, 2015 10:30:00 AM

Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions.

The 1980s saw a flurry of discovery in the field of eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional activators: that the DNA binding domain (DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2), and that DBD and TAs from different systems could be combined effectively (3).

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Topics: Plasmid Technology, Yeast

Plasmids 101: Blue-white Screening

Posted by Jessica Welch on Jun 4, 2015 9:03:00 AM

This post is part of our ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts here.

Now that we have covered antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning reaction. Being able to select for colonies that contain your plasmid is a great start when cloning, but how about being able to choose those that contain plasmid with an insert? Blue-white selection is a widely used method to do just that!

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Topics: Plasmid Elements, Plasmids 101

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