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Sequencing Options for CRISPR Genotyping

Posted by Guest Blogger on Oct 4, 2016 10:30:00 AM

This post was contributed by guest blogger Søren Hough, the Head Science Writer at Desktop Genetics.

One of the most important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR genome editing system you use, there remains a chance that the observed phenotype was caused by an off-target mutation and not an edit in the target gene.

The validation process, also known as CRISPR genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying these connections can help alleviate the reproducibility crisis in biology. It is key to address these concerns as CRISPR use grows across the life sciences and to establish standardized validation techniques for academia, industry, and especially the clinic.

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Topics: CRISPR

Technique: Probe Phage Genomes for Host Binding Proteins

Posted by Guest Blogger on Sep 29, 2016 10:30:00 AM

This post was contributed by guest blogger, Jessica Sacher, a microbiology PhD student at the University of Alberta studying with the Szymanski lab.

Reasons to Study How a Phage Recognizes Its Host

Bacteriophages (viruses that prey on bacteria) may be the most numerous and most diverse biological entities on our planet, but we still know collectively little about how they infect and influence the evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition factors of phages) constitute one of the most popular classes of phage proteins to study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools and therapeutics. In addition, the strategic use of whole phages as therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell.

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Topics: Protocols, Techniques, Microbiology

5 Great Apps for Lab Life

Posted by Guest Blogger on Sep 22, 2016 10:30:00 AM

This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article are those of the guest blogger and do not necessarily represent the views of Addgene.

I think it’s safe to assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate student, I have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that have been educational and great for lab life. These apps prevent you from constantly searching the interwebs for lab resources, and instead conveniently provide them on your smart phone.

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Topics: Lab Tips

Running for Rare Disease, Running for FOP, Running for AJ

Posted by Guest Blogger on Sep 15, 2016 10:30:00 AM

This post was contributed by Kurt Swanson a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA.


When I joined Sanofi/Genzyme nearly three years ago I decided it was time to get in shape. After three kids, I had put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined a gym, and started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based running team called Running for Rare Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money and awareness for rare disease and helps patients find the correct treatments, if available. As part of the team, I started adding mile after mile on my poor, poor legs.

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Topics: Fun, Inside Addgene

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Posted by Guest Blogger on Sep 13, 2016 10:30:00 AM

This post was contributed by guest blogger, David T. Riglar

Advocating for Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston

The Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future of Science, and the MIT Graduate Student Council will be held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts toward positive change in the scientific enterprise and the way it is funded and to learn tangible skills necessary for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman and CEO of the American Association for the Advancement of Science, Rush Holt, followed by a networking reception. Saturday’s workshop will be an advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org/advocating-for-science-boston-2016/.

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Topics: Career, Science Communication, Career Readiness, Mentoring for Scientists

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