This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes ...
This post was contributed by guest blogger Matthew J. Niederhuber, a graduate student at UNC Chapel Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where a protein binds genome-wide, and has been widely ...
This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could ...
This post was contributed by guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows washing away of unbound ...
This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in Alzheimer’s ...
This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were software tools available to biomedical researchers manipulating DNA sequences on a daily basis, many found these tools inadequate for ...
This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. Founder of Casseus Law. Disclaimer: The contents of this post are intended to convey general information only and not to provide legal advice or opinions. The contents of this ...
This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most enjoy is building and telling interesting stories ...
