Beth Kenkel

Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She is particularly interested in science communication and in vitro diagnostics.

Recent Posts

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Posted by Beth Kenkel on Feb 22, 2017 10:30:00 AM

Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing is then used to measure the frequency of each variant during the selection process. Each variant is assigned a functional score based on its library frequency before selection compared to its library frequency after selection. Key to this process is the ability to generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries of mutant plasmids ready for deep mutational scanning.

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Topics: Techniques

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