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Amanda Hazen

Amanda Hazen is the Laboratory Operations Manager at Addgene. In addition to helping with daily laboratory operations, she also works on improving laboratory efficiency through automation.

Recent Posts

Plasmids 101: An Inside Look at NGS Plasmid Quality Control

Posted by Amanda Hazen on Aug 3, 2017 8:49:45 AM

All plasmids coming through Addgene’s doors are now verified by next-generation sequencing (NGS) to provide you with more data. For most plasmids, we are now able to confirm the full insert and backbone sequence. In order to manage and interpret these data, we’ve updated our quality control workflow.  Here’s a peek at how our process has improved now that we’re backed by the power of NGS!

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Topics: Inside Addgene, Plasmids 101

Tips from the Repository Trenches: Using Barcodes to Track Samples

Posted by Amanda Hazen on Oct 6, 2015 10:30:00 AM

Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA in a microcentrifuge tube. Once a sample enters Addgene’s lab, a series of events is triggered. Each step is tracked through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database we use to keep track of and manage all of the samples that come through our doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available for distribution!

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Topics: Inside Addgene, Lab Tips

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